In view of the shortcomings of controlling the quality of oleanolic fruit acid by acid-base non-aqueous solution titration method, and the large error is large, the content of oleanolic fruit acid in oleanolic acid tablets was separated and determined by high performance liquid chromatography. The chromatographic conditions were: the column was ZORBAX SB-C18 column, the mobile phase was methanol-water (90:10), the flow rate was 0.8 mL·min-1, the detection wavelength was 215 nm, and the column temperature was 27 °C. Under this condition, the oleanolic acid was in the range of 0.025 2~2.520 0 mg·mL-1, and the linear relationship was good, r=0.999 7, the average recovery rate of oleanolic fruit acid was 99.4%, peaking at about 13 min, and RSD=1.9%. The quality of the preparation can be effectively controlled.