[1]阮宝玉,赵文恩.高速逆流色谱法分离红色西瓜瓤粗提物中番茄红素[J].郑州大学学报(工学版),2016,37(05):43-46.[doi:10.3969/j.issn.1671-6833.2016.05.009]
　Ruan Baoyu,Zhao Wenen.Isolation of lycopene from the crude extract of red watermelon flesh by high-speed counter-current chromatograghy[J].Journal of Zhengzhou University (Engineering Science),2016,37(05):43-46.[doi:10.3969/j.issn.1671-6833.2016.05.009]
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高速逆流色谱法分离红色西瓜瓤粗提物中番茄红素()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
37
期数:
2016年05期
页码:
43-46
栏目:
出版日期:
2016-11-25

文章信息/Info

Title:
Isolation of lycopene from the crude extract of red watermelon flesh by high-speed counter-current chromatograghy
作者:
阮宝玉; 赵文恩
1.郑州大学化工与能源学院,河南郑州450001;2.食品工业学院食品工业系,越南岘港
Author(s):
Ruan Baoyu1; Zhao Wenen2
1. College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, Henan 450001; 2. Department of Food Industry, College of Food Industry, Da Nang, Vietnam
关键词:
Keywords:
DOI:
10.3969/j.issn.1671-6833.2016.05.009
文献标志码:
A
摘要:
应用高速逆流色谱法(HSCCC)从红色西瓜瓤粗提取物中分离番茄红素.在超声波作用下以v石油醚:v丙酮=2:1作为提取溶剂,从400 g成熟西瓜瓤匀浆中提取粗制番茄红素,至所有色素提取出.然后用vn-正已烷:v乙腈:v二氯甲烷=20:12:7构成的两相溶剂系统进行制备高速逆流色谱分离,从约223 mg粗提物中得到约30 mg番茄红素.用峰面积归一化法计算,产品纯度为98.3％.
Abstract:
Lycopene was separated from the crude extract of red watermelon pulp by high-speed countercurrent chromatography (HSCCC). Under the action of ultrasonic waves, v petroleum ether: v acetone = 2:1 was used as the extraction solvent to extract lycopene from 400 g of mature watermelon pulp homogenate Crude lycopene, until all the pigments are extracted. Then use a two-phase solvent system composed of vn-n-hexane:v acetonitrile:v dichloromethane=20:12:7 for preparative high-speed countercurrent chromatography separation, from about 223 mg About 30 mg lycopene was obtained from the crude extract. Calculated by the peak area normalization method, the product purity was 98.3%.
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