[1]韩秀丽,谷鹏举,方书起,等.Fe3O4磁性壳聚糖微球固定化脂肪酶研究[J].郑州大学学报(工学版),2018,39(04):30-35.[doi:10.13705/j.issn.1671-6833.2018.01.014]
　Han Xiuli,Gu Pengju,Fang Shuqi,et al.Study on Immobilization of Lipase with Fe3O4 Magnetic Chitiosan Micropheres[J].Journal of Zhengzhou University (Engineering Science),2018,39(04):30-35.[doi:10.13705/j.issn.1671-6833.2018.01.014]
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Fe3O4磁性壳聚糖微球固定化脂肪酶研究()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
39
期数:
2018年04期
页码:
30-35
栏目:
出版日期:
2018-07-22

文章信息/Info

Title:
Study on Immobilization of Lipase with Fe3O4 Magnetic Chitiosan Micropheres
作者:
韩秀丽;  谷鹏举; 方书起; 陈俊英
1.郑州大学 化工与能源学院,河南 郑州4500012；生物质炼制技术与装备河南省工程实验室,河南郑州450001
Author(s):
Han Xiuli1; Gu Pengju2; Fang Shuqi1; Chen Junying1
1. School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, Henan; 2. Henan Engineering Laboratory of Biomass Refining Technology and Equipment, Zhengzhou 450001, Henan
关键词:
Keywords:
Magnetic chitosan microspheres;  Azide;  lipase
DOI:
10.13705/j.issn.1671-6833.2018.01.014
文献标志码:
A
摘要:
研究了磁性壳聚糖微球固定化脂肪酶，旨在增加脂肪酶的重复利用率。利用悬浮交联法制备出粒径40-60um的磁性壳聚糖微球，微球通过接枝、叠氮化修饰后用于固定化脂肪酶。通过响应面法考察反应条件对固定化酶的影响，得出最优固定条件：酶浓度4.07mg/mL，反应时间8.41h，反应温度39.3℃，pH值7.0。最优条件下壳聚糖微球实际载酶量为64.4mg/g，与预测值相接近，证明该方法可以用于固定化脂肪酶。
Abstract:
In order to increase the utilization rate of lipase, the immobilization of lipase by azidation magnetic chitosan was investigated. The magnetic chitosan microspheres with particle size of 40~60 µm were prepared by suspension croddlinking method. The microspheres were modified by grafting and azide. Response surface methodology was used to investigate the effect of reaction conditions on the immobilized enzyme, and the optimum conditions for lipase immobilization were concentration of enzyme 4 mg/ml, reaction time 8.4h, reaction temperature 39.3ºc and the ph 7.0. In the optimum conditions, the immobilized enzyme amount of magnetic chitosan microsheres was 64.4 mg/g, with the actual value showing a good fit to the predicted value. The results suggested that this method could be uaed to immobilized lipase.
更新日期/Last Update: 2018-07-25