[1]毛陆原,罗成果,李玉娜,等.空气消毒剂过氧乙酸含量的酶催化光度法测定[J].郑州大学学报(工学版),2005,26(01):73-78.[doi:10.3969/j.issn.1671-6833.2005.01.019]
Mao Luyuan,Luo Fruit,LI Yuna,et al.Enzyme-catalyzed photometric determination of peracetic acid content in air disinfectants[J].Journal of Zhengzhou University (Engineering Science),2005,26(01):73-78.[doi:10.3969/j.issn.1671-6833.2005.01.019]
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空气消毒剂过氧乙酸含量的酶催化光度法测定(
)
《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]
- 卷:
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26卷
- 期数:
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2005年01期
- 页码:
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73-78
- 栏目:
-
- 出版日期:
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1900-01-01
文章信息/Info
- Title:
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Enzyme-catalyzed photometric determination of peracetic acid content in air disinfectants
- 作者:
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毛陆原; 罗成果; 李玉娜; 等.
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郑州大学材料工程学院,河南,郑州,450052, 郑州大学材料工程学院,河南,郑州,450052;河南省化学生物和有机化学重点实验室,河南郑州,450052
- Author(s):
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Mao Luyuan; Luo Fruit; LI Yuna; etc
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- 关键词:
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过氧乙酸; 辣根过氧化物酶; 在线预富集; 空气消毒; 酶法分析
- Keywords:
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- DOI:
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10.3969/j.issn.1671-6833.2005.01.019
- 文献标志码:
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A
- 摘要:
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过氧乙酸(PAcA)室内作消毒剂时,在空气中的含量很低,用通常的化学分析方法很难检测到.通过用过氧乙酸以喷雾法和熏蒸法进行空气消毒,用气体吸收管现场采样,以酶催化分光光度法测定室内环境中的微量过氧乙酸消毒剂的含量.实验证明:PAcA在辣根过氧化物酶(HRP)催化作用下,当磷酸盐缓冲液存在时能和苯酚、4-氨基安替比林发生Trinder反应.与过氧化氢的酶催化分光光度法相似,35℃生成的醌式染料30 min显色完全,在505nm处有最大吸收,对应该波长的吸光系数为12.72L/(mmol·cm),测量过氧化物
- Abstract:
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When peracetic acid (PAcA) is used as a disinfectant indoors, the content in the air is very low and it is difficult to detect with conventional chemical analysis methods. The content of trace peracetic acid disinfectant in indoor environment was determined by enzyme-catalyzed spectrophotometry by air disinfection by spray method and fumigation method, and on-site sampling with gas absorption tube. Experiments show that PAcA can react with phenol and 4-aminoantipyrine when phosphate buffer is present under the catalysis of horseradish peroxidase (HRP). Similar to the enzyme-catalyzed spectrophotometry of hydrogen peroxide, the quinone dye generated at 35 °C has complete color development for 30 min, has maximum absorption at 505 nm, and the absorbance coefficient of the corresponding wavelength is 12.72L/(mmol·cm), and the peroxide is measured
更新日期/Last Update:
1900-01-01