[1]孙 鑫,王献丽,马晓吉,等.PB 试验-响应面法优化黄芩提取物的纯化工艺[J].郑州大学学报(工学版),2023,44(04):107-112,119.[doi:10.13705/j.issn.1671-6833.2023.01.002]
　SUN Xin,WANG Xianli,MA Xiaoji,et al.Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology[J].Journal of Zhengzhou University (Engineering Science),2023,44(04):107-112,119.[doi:10.13705/j.issn.1671-6833.2023.01.002]
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PB 试验-响应面法优化黄芩提取物的纯化工艺()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
44
期数:
2023年04期
页码:
107-112,119
栏目:
出版日期:
2023-06-01

文章信息/Info

Title:
Optimization of Purification Process of Scutellaria baicalensis Extract by PB Test with Response Surface Methodology
作者:
孙 鑫1; 王献丽1; 马晓吉1; 邓芳芳2; 潘成学1; 2
1.郑州大学 药学院,河南 郑州 450001, 2.河南郑大杰诺医药技术有限公司,河南 郑州 450001.

Author(s):
SUN Xin1;  WANG Xianli1;  MA Xiaoji1; DENG Fangfang2;  PAN Chengxue1; 2
1.School of Pharmacy, Zhengzhou University, 450001, Zhengzhou, Henan, 2.Henan Zhengda Jienuo Pharmaceutical Technology Co., Ltd., Henan Zhengzhou 450001.
关键词:
黄芩苷;  纯化;  Plackett-Burman 试验;  Box-Behnken 响应面试验;  质量源于设计
Keywords:
baicalin;  purification;  Plackett-Burman test;  Box-Behnken response surface test;  quality by design
分类号:
R284.2
DOI:
10.13705/j.issn.1671-6833.2023.01.002
文献标志码:
A
摘要:
以黄芩苷得率和黄芩苷纯度为评价指标,采用酸沉法、碱溶酸沉法、重结晶法、药典纯化方法对 黄芩苷进行纯化,确定最佳纯化方法为碱溶酸沉法。 运用 Plackett-Burman 试验得到碱溶酸沉法中的关 键工艺参数,通过 Box-Behnken 响应面试验建立关键工艺参数和关键评价指标之间的回归模型,获取设 计空间并进行验证。 筛选得到的关键参数为药液浓度、碱溶温度、碱溶 pH、酸沉温度和酸沉 pH,在满足 黄芩苷得率和纯度均大于 85%时,得到操作空间碱溶温度为 40 ℃ 、碱溶 pH 为 8. 0、酸沉温度为 80 ℃ 、 药液浓度为 11. 53 ~ 14 mg·mL- 1 、酸沉 pH 为 1. 17 ~ 2. 33。 验证结果表明:设计空间稳定可靠,可为工业 化生产黄芩提取物提供参考。
Abstract:
Taking the yield and purity of baicalin as evaluation indicators, the acid precipitation method, alkali-soluble acid precipitation method, recrystallization method and pharmacopeia purification method were used to purify baicalin, and the best purification method was determined as alkali-soluble acid precipitation method. Plackett-Burman test was used to obtain the key process parameters in the alkali-soluble acid precipitation method, and the regression model between the key process parameters and the key evaluation indicators was established by the Box-Behnken response surface test, and the design space was obtained and verified. The key parameters obtained by screening were the liquid concentration, alkali solution temperature, alkali solution pH, acid precipitation temperature and acid precipitation pH. When the yield and purity of baicalin were both greater than 85%, the operating space were: the alkali solution temperature was 40 ℃ , the pH of alkali solution was 8. 0, the temperature of acid precipitation was 80 ℃ , the concentration of liquid medicine was 11. 53 ~ 14 mg·mL - 1 , and the pH of acid precipitation was 1. 17 ~ 2. 33. The verification results show that the design space was stable and reliable, and it could provide a reference for industrial production of Scutellaria baicalensis extract.
更新日期/Last Update: 2023-07-01