Based on the database and information management technology, the information related to measurement equipment, measurement methods and influencing factors of measurement uncertainty assessment management was analyzed, the design method and interface technology of open database were studied, and the basic database of PUMA was constructed. Based on the knowledge of artificial intelligence expert system, the internal regularity of the basic database is analyzed, the relationship between the PUMA application system and the basic database is studied, and the call of the PUMA application system to the basic database according to certain rules is realized.