[1]张茜,芮瑞,李佩佩,等.草乌多糖金属配合物的合成、表征及其抗癌活性的研究[J].郑州大学学报(工学版),2016,37(03):36.[doi:10.13705/ j.issn.1671 -6833.2016.03.008]
　ZHANG Xi,RUI Rui,Ll Peipei,et al.Preparation，Characterization, and Anticancer Evaluation of Inner MongoliaRadix Aconiti Kusenezoffi Polysaccharide Metal Complexes[J].Journal of Zhengzhou University (Engineering Science),2016,37(03):36.[doi:10.13705/ j.issn.1671 -6833.2016.03.008]
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草乌多糖金属配合物的合成、表征及其抗癌活性的研究()
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《郑州大学学报(工学版)》[ISSN:1671-6833/CN:41-1339/T]

卷:
37
期数:
2016年03期
页码:
36
栏目:
出版日期:
2016-05-10

文章信息/Info

Title:
Preparation，Characterization, and Anticancer Evaluation of Inner MongoliaRadix Aconiti Kusenezoffi Polysaccharide Metal Complexes
作者:
张茜; 芮瑞; 李佩佩; 冯翠宁; 雷强; 龙跃
1.郑州大学化学与分子工程学院，河南郑州450001;2.锡林郭勒盟蒙医医院内窥镜科，内蒙古锡林浩特026000
Author(s):
ZHANG Xi1; RUI Rui2; Ll Peipei1; FENG Cuining1; LEl Qiang1; LONG Yue1
1.College of Chemistry and Molecular Engineering , Zhengzhou University , Zhengzhou 450001 , China; 2. Department of Endo-scope，Xilinguole Meng Mongolian General Hospital，Xilinhaote 026000 , China
关键词:
草乌; 多糖金属配合物; 制备; 表征; 抗癌活性
Keywords:
radix aconiti kusnezoffii; polysaccharide metal complex;  preparation; characterization;  antican-cer activity
分类号:
O629.1
DOI:
10.13705/ j.issn.1671 -6833.2016.03.008
文献标志码:
A
摘要:
以水为提取溶剂从草乌中提取草乌多糖(RPS) ,确定了多糖提取的最佳工艺条件:提取温度90℃;提取时间4 h;料液比1:50,并以草乌多糖为配体,制备了4种多糖金属配合物(RPS-Ca、RPS-Zn、RPS-Cu,RPS-Fe).采用MTT(噻唑蓝)法,以配体草乌多糖为对照,测定了4种多糖金属配合物对肝癌细胞(HepG2)、乳腺癌细胞(MCF-7)和结肠癌细胞(HT-29)的抑制作用.实验结果显示,草乌多糖铜配合物对3种癌细胞表现出最强的抑制作用.草乌多糖铜配合物的结构经紫外光谱(UV)、红外光谱(IR)、圆二色谱( CD)、电镜扫描(SEM)和热重分析(TGA)得到表征.
Abstract:
By using water as extraction reagent,the polysaccharides in Radix Aconiti Kusnezoffi were extrac-ted.The results showed that optimum extraction condition was 90℃,4 h,and liquid to material ratio 1:50.Four polysaccharide complexes were prepared using four metal ions(Ca2+,Zn3+,Cu2+,Fe3+) and polysaccha-rides from Radix Aconiti Kusnezoffi,respectively. All complexes were evaluated for biological activity againstthree human cancer cell lines (HepG2,MCF-7 and HT-29) by the standard MTT method, and the resultsshowed that PRS-Cu possessed the highest growth inhibitory effects.The complex was characterized by UV,IR,D,SEM, and TGA.
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