ESI MS研究黄酮含磷衍生物与溶菌酶的竞争作用-《郑州大学学报(工学版)》

文章信息/Info

Title:: Study on Competitive Interaction of Lysozyme and Chrysin PhosphorylatedDerivatives by ESI Mass Spectrometry

作者:: 陈晓岚; 李慧; 袁金伟; 等.; 郑州大学,化学系,河南省化学生物与有机化学重点实验室,河南,郑州,450001, 中原工学院河南,郑州,450007, 郑州大学,化学系,河南省化学生物与有机化学重点实验室,河南,郑州,450001;河南工业大学,化学化工学院,河南,郑州,450002

Author(s):: CHEN Xiaolan; LI Hui; YUAN Jinwei; etc; 1．Department of Chemistry，Zhengzhou University，Key Laboratory of Chemistry Biology and Organic Chemistry，Zhengzhou450001，China；2．Zhongyuan University of Technology，Zhengzhou 450007，China；3．School of Chemistry＆Chemical Engi—neering，Henan University of Technology，Zhengzhou 450002，China．

Keywords:: ESI mass spectrometry; Flavonoid-7-phosphorylated amino acid esters; Flavonoid-7-diisopropyl phosphate; Lysozyme; Competitive role

摘要:: 采用电喷雾质谱(ESI MS)技术,研究了黄酮-7-磷酰化丙氨酸酯(a)、黄酮-7-磷酰化缬氨酸酯(b)、黄酮-7-磷酰化亮氨酸酯(c)、黄酮-7-磷酰化异亮氨酸酯(d)分别与黄酮-7-二异丙基磷酸酯(e)、溶菌酶形成的三体系混合溶液小分子与蛋白大分子的弱相互作用.实验结果表明,在这个三体系的混合溶液中,黄酮-7-磷酰化氨基酸酯a、b、c、d与黄酮-7-二异丙基磷酸酯e相比,与溶菌酶具有更强的弱相互作用能力或亲和力.

Abstract:: In the competition experiment，the non—covalent complexes was studied by electrospray ionizationmass spectrometry between the same protein lysozyme and two different kinds of chrysin phosphorylated derivatives，such as Ca and Ce，Cb and Ce，Cc and Ce，Cd and Ce．It was found that，in spite of all of compoundscontaining phosphoryl group，there were differences among them．The interaction lysozyme and chrysin一7一phosphated amino acid esters(a，b，c，d)was stronger than that of chrysin一7一diisopropyl phosphate(e)．